The Wong Lab

Single-molecule Force Studies



C.H. Hansen, D. Yang, M.A. Koussa, W.P. Wong, Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection, Proc. Natl. Acad. Sci. USA (2017). [link] [pdf] [supplementary info]

D. Yang, W.P. Wong, Repurposing a benchtop centrifuge for high-throughput single-molecule force spectroscopy, Methods in Molecular Biology: Single Molecule Analysis: Methods and Protocols, Second Edition 1665 (in press).

H. Fu#, Y Jiang#, D. Yang, F. Scheiflinger, W.P. Wong*^, T.A. Springer*^, Flow-induced elongation of von Willebrand factor precedes tension-dependent activation, Nature Communications 8, 324 (2017) (*#equal contribution, ^co-corresponding/senior authors). [link] [pdf] [supplementary info]

D. Yang, W.P. Wong, Small but mighty: Nanoparticles probe cellular signaling pathways, Developmental Cell 37 (5), 397-398 (2016). [link]

D. Yang, A. Ward, K. Halvorsen, W.P. Wong, Multiplexed single-molecule force spectroscopy using a centrifuge, Nature Communications 7, 11026 (2016). [link] [pdf] [supplementary info]

    Featured as a Research Highlight in Nature Methods:

  1.     “Putting a spin on high-throughput force spectroscopy,” Nature Methods 13,
          396 (2016). [link]

M.A. Koussa*, K. Halvorsen*, A. Ward, W.P. Wong, DNA Nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis, Nature Methods (2015). (*equal contribution) [link] [pdf] [protocol] [videos] [author file]

A.K. Drukier, Ch. Cantor, M. Chonofsky, G.M. Church, R.L. Fagaly, K. Freese, A. Lopez, T. Sano, C. Savage, W.P. Wong, New class of biological detectors for WIMPs, Int. J. Mod Phys B, 29 (19), 1443007 (2014). [pdf]

M.A. Koussa, M. Sotomayor, and W.P. Wong, Protocol for sortase-mediated construction of DNA-protein hybrids and functional nanostructures, Methods, 67 (2), 134-141 (2014). [pdf] [link]

W. Zhao, C.H. Cui, S. Bose, D. Guo, C. Shen, W.P. Wong, K. Halvorsen, O.C. Farokhzad, G.S.L. Teo, J.A. Phillips, D.M. Dorfman, R. Karnik, & J.M. Karp, A bioinspired multivalent DNA network for capture and release of cells, Proc. Natl. Acad. Sci. USA, 109 (48), 19626-19631 (2012). [link]

J. Pelletier*, K. Halvorsen*, B.-Y. Ha, R. Paparcone, S. Sandler, C. Woldringh, W.P. Wong^, & S. Jun^, Physical manipulation of the Escherichia coli chromosome reveals its soft nature, Proc. Natl. Acad. Sci. USA 109 (4), E2649-E2656 (2012). (*equal contribution, ^co-corresponding/senior authors). [pdf] [link]

    Featured as a Research Highlight in Nature Methods and PNAS:

  1.     “Soft bacterial chromosomes,” Nature Methods 9, 1047 (2012). [pdf] [link]

  2.     “Under pressure, bacterial chromosomes mimic soft springs,” PNAS 109
          (2012). [link]

K. Halvorsen and W.P. Wong, Binary DNA Nanostructures for Data Encryption. PLoS ONE 7 (9), e44212 (2012). [link]

K. Halvorsen, D. Schaak, and W.P. Wong, Nanoengineering a single-molecule mechanical switch using DNA self-assembly, Nanotechnology 22 (49), 494005 (2011). [pdf] [link]

K. Halvorsen, W.P. Wong, “Massively parallel single-molecule manipulation using centrifugal force,” Biophysical Journal 98 (11), L53-L55 (2010). [pdf]

    Featured as a Research Highlight in Nature and Nature Methods:

  1.     “Biophysics: Molecular carnival ride,” Nature 465, 669 (2010). [link] [pdf]

  2.     “High-throughput single-molecule force spectroscopy,” Nature Methods 7
           489 (2010). [link] [pdf]

D. Cheng, K. Halvorsen, W.P. Wong, “High-precision microsphere sorting using velocity sedimentation,” Reviews of Scientific Instruments 81, 026106 (2010). [pdf]

X. Zhang*, K. Halvorsen*, C.-Z. Zhang, W.P. Wong^, and T.A. Springer^, “Mechanoenzymatic cleavage of the ultralarge vascular protein, von Willebrand Factor,” Science 324 (5932), 1330-1334 (2009). (*equal contribution,
^co-corresponding/senior authors) [pdf]

    This research was highlighted in Science and Nature Medicine:

  1.     “Force signaling in Biology,” J. Christof, M. Gebhardt, Matthias Rief, Science
           324, 1278-1280 (2009). [link] [pdf]

  2.     “Vascular disorders: Biological shear bolt,” Nature Medicine 7 15, 738-739
          (2009). [link] [pdf]

E. Evans, K. Halvorsen, K. Kinoshita, and W.P. Wong, “A new approach to analysis of single molecule force measurements,” in Handbook of Single Molecule Biophysics, P. Hinterdorfer, ed., Springer (2009). [pdf] [link]

W.P. Wong*^ and K. Halvorsen*, "Beyond the frame rate: Measuring high-frequency fluctuations with light intensity modulation," Optics Letters 34 (3), 277-279 (2009). (*equal contribution, ^corresponding author) [pdf]

V. Heinrich*^, W.P. Wong*, K. Halvorsen, and E. Evans, “Imaging biomolecular interactions by fast three-dimensional tracking of laser-confined carrier particles,” Langmuir 24, 1194-1203 (2008).  (*equal contribution, ^corresponding author) [pdf]

K. Halvorsen, “Probing weak single-molecule interactions: development and demonstration of a new instrument,” Ph. D Thesis (advisor: E. Evans), Department of Biomedical Engineering, Boston University, USA (2007). [pdf]

W.P. Wong*^ and K. Halvorsen*, “The effect of integration time on fluctuation measurements: calibrating an optical trap in the presence of motion blur,” Optics Express 14 (25), 12517-12531 (2006). (*equal contribution, ^corresponding author) [pdf]

W.P. Wong, “Exploring single-molecule interactions through 3D optical trapping and tracking: from thermal noise to protein refolding,” Ph. D Thesis (advisors: D.R. Nelson and E. Evans), Department of Physics, Harvard University, USA (2006). [pdf]

W.P. Wong and E. Evans, “Rare returns on lost effort,” Nature 437, 198-199 (2005).[pdf]

W.P. Wong, V. Heinrich, and E. Evans, “Exploring reaction pathways of single-molecule interactions through the manipulation and tracking of a potential-confined microsphere in three dimensions,” Mat. Res. Soc. Symp. Proc. 790, P5.1.1-P5.1.12 (2004). [pdf]

W.P. Wong, “Modelling a Tethered Polymer Loop: Shape and thickness compressibility,” Master’s Thesis, The University of British Columbia, Canada (2001). [pdf]